別名:
人IgE全長蛋白質
研究領域:
免疫學
詳述
抗體應用:
未定義
蛋白長度Full length protein
描述
性質Natural
來源Native
氨基酸序列
種屬Human
額外的序列信息Observed band of ~185kDa on SDS-PAGE
技術指標
Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
應用
SDS-PAGE
ELISA
Immunocytochemistry
內毒素水平< 25.000 Eu/mg
純度> 90 % by SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
形式Liquid
補充說明
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
Less batch-to-batch variation
Higher degree of purity (>90%)
A more uniform product (monoclonal and not polyclonal)
IgE has been used in a variety of cell stimulation assays, including the following cell types:
Human airway smooth muscle (HASM) cells
mast cells (MC)
Human bronchial/tracheal smooth muscle (B/TSM) cells
acute myelogenous leukemia (AML) blasts
Human neutrophils
This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
1 mg of ab65866 is 380,000± 50,000 IU/ml.
濃度100 |
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