IntroductionThe serine-protease-inhibitor antithrombin III (AT III), the most important natural inhibitor of thrombin activity, has been shown to exert marked anti-inflammatory properties and proven to be efficacious in experimentalmodels of sepsis, septic shock, and disseminated intravascular coagulation (1). It has often been recommended for the therapy of septic patients as it provides anticoagulant and anti-inflammatory actions (2). Antithrombin III (AT III) deficiency is a rare hereditary disease that predisposes to throm-boembolic complications (3). AT III levels are positively correlated with plasma total cholesterol levels, plasma low-density lipoprotein cholesterol levels, plasma triglycerides and D-dimer levels (4). Principal of the AssayThe AssayMax AT III ELISA kit is designed for detection of human AT III in plasma, and serum. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures AT III in 4 hours. A polyclonal antibody specific for AT III has been pre-coated onto a microplate. AT III in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for AT III, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured. Caution and Warning
• This kit is for research use only.
• The kit should not be used beyond the expiration date.
• The Stop Solution is an acid solution.
Reagents
• AT III Microplate:A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human AT III.
• Sealing Tapes:Each kit contains 3 pre-cut, pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay.
• AT III Standard:Human AT III in a buffered protein base (12 μg, lyophilized). |
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