重組人GM-CSF(大腸桿菌表達(dá))
中文名稱(chēng):重組人GM-CSF(大腸桿菌表達(dá))英文名稱(chēng):Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor產(chǎn)品規(guī)格:10μg|50μg|500μg|1mg發(fā)貨周期:1~3天
GM-CSF質(zhì)量控制:>GM-CSF制劑:凍干品
GM-CSF保存:
凍干蛋白可在-20℃長(zhǎng)期保存,室溫也可穩(wěn)定保存3周。
復(fù)溶蛋白溶液可在4~7℃保存2~7天。
復(fù)溶后建議分裝凍存在-20℃,可保存3個(gè)月,應(yīng)避免反復(fù)凍融。
GM-CSF復(fù)溶:
1、試劑開(kāi)蓋前請(qǐng)先離心。
2、復(fù)溶濃度應(yīng)大于100 μg/ml.
3、凍干蛋白溶于ddH2O中
4、復(fù)溶后根據(jù)使用量分裝保存,避免反復(fù)凍融。
關(guān)于GM-CSF:
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines |
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